Fluorescence detection of natural RNA using rationally designed “clickable” oligonucleotide probes

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Fluorescence detection of natural RNA using rationally designed “clickable” oligonucleotide probes

Interdisciplinary Nanoscience Center (iNAN and Genetics, Aarhus University, Gustav W E-mail: [email protected]; Tel: +45 28992086 Nucleic Acid Center, Department of Physics Southern Denmark, DK-5230 Odense M, Denma † Electronic supplementary information purication and characterization of the limit of target detection calculations, an targeting complementary and singly-mis 10.1039/c4ra07165d Cite thi...

متن کامل

Rationally-designed fluorescent lysine riboswitch probes.

Two fluorescent lysine amide analogs, in which the carboxyl end of lysine was covalently attached to dansyl or NBD groups through an ethylene glycol-based linker, were rationally designed and synthesized. Both probes showed high binding affinity to the lysine riboswitch in vitro and their fluorescence intensities decreased by riboswitch binding.

متن کامل

Substantial background reduction in ligase-based apoptosis detection using newly designed hairpin oligonucleotide probes.

A ligase-based assay for selective detection of apoptosis in tissue sections using oligonucleotide probes was recently reported (1). This assay detects apoptosis when it is accompanied by internucleosomal DNA cleavage with the production of multiple doublestrand breaks and electrophoretic ladder-type DNA fragmentation. Detection of double-strand breaks in apoptotic DNA in this assay is by ligat...

متن کامل

Enzyme-Free Detection of Mutations in Cancer DNA Using Synthetic Oligonucleotide Probes and Fluorescence Microscopy

BACKGROUND Rapid reliable diagnostics of DNA mutations are highly desirable in research and clinical assays. Current development in this field goes simultaneously in two directions: 1) high-throughput methods, and 2) portable assays. Non-enzymatic approaches are attractive for both types of methods since they would allow rapid and relatively inexpensive detection of nucleic acids. Modern fluore...

متن کامل

Cellular Uptake and Localization of Polymyxins in Renal Tubular Cells Using Rationally Designed Fluorescent Probes.

Polymyxins are cyclic lipopeptide antibiotics that serve as a last line of defense against Gram-negative bacterial superbugs. However, the extensive accumulation of polymyxins in renal tubular cells can lead to nephrotoxicity, which is the major dose-limiting factor in clinical use. In order to gain further insights into the mechanism of polymyxin-induced nephrotoxicity, we have rationally desi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: RSC Adv.

سال: 2014

ISSN: 2046-2069

DOI: 10.1039/c4ra07165d